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bdev:nucleic_acid_extraction [2020/11/29 19:21] richard |
bdev:nucleic_acid_extraction [2021/08/16 22:22] (current) |
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Protocol | Protocol | ||
- | - .4cm^2 whatman paper cut and placed into sterile 1.5mL Eppendorf tubes (sandwiched standing perpendicular to the ground toward the bottom of the tube) | + | - 0.4cm^2 whatman paper cut and placed into sterile 1.5mL Eppendorf tubes (sandwiched standing perpendicular to the ground toward the bottom of the tube) |
- 500uL Lysis buffered added to tubes | - 500uL Lysis buffered added to tubes | ||
- 100ul O/N E.coli BL21 in LB broth added to tubes | - 100ul O/N E.coli BL21 in LB broth added to tubes |