Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1164

Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1168

Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1171

Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1172

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

--- bdev:pcr_notes [2020/11/14 00:56]
richard
+++ bdev:pcr_notes [2021/08/16 22:22] (current)
@@ Line 1: / Line 1: @@
 ====== PCR notes ======
+To make stronger signal, I will mix all the primer targets together (IL10, Hdac3, Ccl3, beta-actin)
+Here is signal
+{{ :bdev:pcr_sanity.png?400 |}}
+| Components | Volume [µL] |
+| Primer mix | 40µL |
+| cDNA | 20µL |
+| water | 40µL |
+| Master mix | 100µL |
+Here is negative control
+| Components | Volume [µL] |
+| Primer mix | 40µL |
+| cDNA | 0µL |
+| water | 60µL |
+| Master mix | 100µL |
 ----
-Deepak kit
+Negative control PCR stock (10 rxns)
+| Components | Volume [µL] |
+| Primer mix | 10µL |
+| cDNA | 0µL |
+| water | 90µL |
+| Master mix | 100µL |
+----
+PCR stock (10 rxns)
+| Components | Volume [µL] |
+| Primer mix | 10µL |
+| cDNA | 20µL |
+| water | 70µL |
+| Master mix | 100µL |
+----
+Deepak kit
+{{:bdev:pcr_recipe_deepak.png?400 |}}
+{{ :bdev:thermocycling.png?400 |}}
+----
 |||
 | Components | Volume [µL] |
-| Taqman Mix | ?µl |
+| Primer mix | ?µl |
-| [[https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/taqman-real-time-master-mixes/taqman-fast-advanced-master-mix.html|Primer Mix]] | ?µl |
+| [[https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/taqman-real-time-master-mixes/taqman-fast-advanced-master-mix.html|Master Mix (2x)]] | 10µl |
 | Sample | 1 µl  |
 | Total volume [µL] | 20µl |
-https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/taqman-real-time-master-mixes/taqman-fast-advanced-master-mix.html
+Primers (Don't know corresponding code, yet)
+  - IL10
+  - Hdac5
+  - Ccl3
+  - beta-actin
+Primer ids
+  - 0208661104
+  - 0374221766
+  - 0347861862
+  - 0367609480
+|{{ :bdev:pcr_primers.png?200 |}}|{{ :bdev:applied_biosystems_taqman_pcr.png?200 |}}|
-{{ :bdev:pcr_primers.png?200 |}}
-{{ :bdev:applied_biosystems_taqman_pcr.png?200 |}}

labautobox

User Tools

Site Tools

Differences

Page Tools