bdev:pcr_notes
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bdev:pcr_notes [2020/11/25 19:35] richard |
bdev:pcr_notes [2021/08/16 22:22] (current) |
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| ====== PCR notes ====== | ====== PCR notes ====== | ||
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| + | To make stronger signal, I will mix all the primer targets together (IL10, Hdac3, Ccl3, beta-actin) | ||
| + | |||
| + | Here is signal | ||
| + | |||
| + | |||
| + | {{ : | ||
| + | |||
| + | |||
| + | | Components | Volume [µL] | | ||
| + | | Primer mix | 40µL | | ||
| + | | cDNA | 20µL | | ||
| + | | water | 40µL | | ||
| + | | Master mix | 100µL | | ||
| + | |||
| + | |||
| + | Here is negative control | ||
| + | |||
| + | |||
| + | | Components | Volume [µL] | | ||
| + | | Primer mix | 40µL | | ||
| + | | cDNA | 0µL | | ||
| + | | water | 60µL | | ||
| + | | Master mix | 100µL | | ||
| + | |||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| Negative control PCR stock (10 rxns) | Negative control PCR stock (10 rxns) | ||
bdev/pcr_notes.1606332950.txt.gz · Last modified: 2021/08/16 22:21 (external edit)
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