bdev:pcr_notes
Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1164
Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1168
Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1171
Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1172
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
bdev:pcr_notes [2020/11/25 19:41] richard |
bdev:pcr_notes [2021/08/16 22:22] (current) |
||
|---|---|---|---|
| Line 2: | Line 2: | ||
| To make stronger signal, I will mix all the primer targets together (IL10, Hdac3, Ccl3, beta-actin) | To make stronger signal, I will mix all the primer targets together (IL10, Hdac3, Ccl3, beta-actin) | ||
| + | |||
| + | Here is signal | ||
| + | |||
| + | |||
| + | {{ : | ||
| + | |||
| | Components | Volume [µL] | | | Components | Volume [µL] | | ||
| | Primer mix | 40µL | | | Primer mix | 40µL | | ||
| | cDNA | 20µL | | | cDNA | 20µL | | ||
| + | | water | 40µL | | ||
| + | | Master mix | 100µL | | ||
| + | |||
| + | |||
| + | Here is negative control | ||
| + | |||
| + | |||
| + | | Components | Volume [µL] | | ||
| + | | Primer mix | 40µL | | ||
| + | | cDNA | 0µL | | ||
| | water | 60µL | | | water | 60µL | | ||
| | Master mix | 100µL | | | Master mix | 100µL | | ||
| + | |||
| + | |||
| + | ---- | ||
bdev/pcr_notes.1606333269.txt.gz · Last modified: 2021/08/16 22:21 (external edit)
Page Tools
Except where otherwise noted, content on this wiki is licensed under the following license: GNU Free Documentation License 1.3
