bdev:precigenome_protocol
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| to deposit samples with the pipette directly into the reaction mix in PCR tubes. Cover each well, centrifuge at 2000 rpm for 10 seconds, and place into Applied Biosystems ABI 7500 real-time | to deposit samples with the pipette directly into the reaction mix in PCR tubes. Cover each well, centrifuge at 2000 rpm for 10 seconds, and place into Applied Biosystems ABI 7500 real-time | ||
| RT-PCR system and record the exact location of controls and each specimen. | RT-PCR system and record the exact location of controls and each specimen. | ||
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| + | - Stage 1: 50°C for 15 min, 1 cycle | ||
| + | - Stage 2: 95°C for 1 min, 1 cycle | ||
| + | - Stage 3: 95°C for 15 sec, 60°C for 30 sec, 45 cycles | ||
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bdev/precigenome_protocol.1604680364.txt.gz · Last modified: 2021/08/16 22:21 (external edit)
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