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pcrmon [2020/11/24 22:32]
richard |
pcrmon [2021/08/16 22:22] (current)
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| Portable PCR system for decentralized testing applications. | Portable PCR system for decentralized testing applications. |
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| ===== Pipetting setup ===== | ===== Pipetting setup for PCR ===== |
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| <figure> | <figure> |
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| <figure> | <figure> |
| {{ ::accurately_aspirate_pcr_reaction_mix.png?400|Accurately aspirating microliter volumes of reaction mix}}<caption>**In order to transfer the reaction from the source well into the PCR test tube, you need to aspirate then dispense the sample. This example demonstrates how it is easy to accurate aspirate microliter volumes of reaction mix (in this action the volume aspirated is 10µl). In order to aspirate, first you squeeze the tubing at the top of the pipette using your index finger and thumb in order to reduce the volume in the pipette, then you move the pipette into the tube containing the reaction mix that you want to aspirate and carefully reduce the squeezing pressure in order to accurate aspirate the volume that reaches the marketing. Then you move the pipette tip to the empty PCR tube and dispense the volume.**</caption> | {{ ::accurately_aspirate_pcr_reaction_mix.png?400|Accurately aspirating microliter volumes of reaction mix}}<caption>**In order to transfer the reaction from the source well into the PCR test tube, you need to aspirate then dispense the sample. This example demonstrates how it is easy to accurately aspirate microliter volumes of reaction mix (in this action the volume aspirated is 10µl). In order to aspirate, first, you squeeze the tubing at the top of the pipette using your index finger and thumb in order to reduce the volume in the pipette, then you move the pipette into the tube containing the reaction mix that you want to aspirate and carefully reduce the squeezing pressure in order to create suction to aspirate the volume enough to reach the marketing. Then you move the pipette tip to the empty PCR tube and dispense the volume.**</caption> |
| </figure> | </figure> |
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| <figure> | <figure> |
| {{ ::accurately_aspirate_mineral_oil_for_pcr.png?400|Accurately aspirating mineral oil}}<caption>**Because the PCRmon thermal block does not have a heated like, mineral oil is needed in order to keep the volume of the reaction mix at the bottom of the PCR tube during thermocycling. The smaller pipettes used for aspirating the reaction mix have too small of an orifice for this relatively viscous mineral oil so a larger bore pipette tip is better for this. The volume of the mineral oil added to the reaction mix is not as critical, you can aspirate the total amount of volume (just squeeze and release entirely when the pipette tip is in the mineral oil), this produces around 30μl of mineral oil in the tube.**</caption> | {{ ::accurately_aspirate_mineral_oil_for_pcr.png?400|Accurately aspirating mineral oil}}<caption>**Because the PCRmon thermal block does not have a heated lid, mineral oil is needed in order to keep the volume of the reaction mix at the bottom of the PCR tube during thermocycling. The smaller pipettes used for aspirating the reaction mix have too small of an orifice for this relatively viscous mineral oil so a larger bore pipette tip is better for this. The volume of the mineral oil added to the reaction mix is not as critical, you can aspirate the total amount of volume (just squeeze and release entirely when the pipette tip is in the mineral oil), this produces around 30μl of mineral oil in the tube.**</caption> |
| </figure> | </figure> |
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