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pcrmon [2020/11/24 22:33]
richard |
pcrmon [2021/08/16 22:22] (current)
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| Portable PCR system for decentralized testing applications. | Portable PCR system for decentralized testing applications. |
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| ===== Pipetting setup ===== | ===== Pipetting setup for PCR ===== |
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| <figure> | <figure> |
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| <figure> | <figure> |
| {{ ::accurately_aspirate_pcr_reaction_mix.png?400|Accurately aspirating microliter volumes of reaction mix}}<caption>**In order to transfer the reaction from the source well into the PCR test tube, you need to aspirate then dispense the sample. This example demonstrates how it is easy to accurate aspirate microliter volumes of reaction mix (in this action the volume aspirated is 10µl). In order to aspirate, first you squeeze the tubing at the top of the pipette using your index finger and thumb in order to reduce the volume in the pipette, then you move the pipette into the tube containing the reaction mix that you want to aspirate and carefully reduce the squeezing pressure in order to accurate aspirate the volume that reaches the marketing. Then you move the pipette tip to the empty PCR tube and dispense the volume.**</caption> | {{ ::accurately_aspirate_pcr_reaction_mix.png?400|Accurately aspirating microliter volumes of reaction mix}}<caption>**In order to transfer the reaction from the source well into the PCR test tube, you need to aspirate then dispense the sample. This example demonstrates how it is easy to accurately aspirate microliter volumes of reaction mix (in this action the volume aspirated is 10µl). In order to aspirate, first, you squeeze the tubing at the top of the pipette using your index finger and thumb in order to reduce the volume in the pipette, then you move the pipette into the tube containing the reaction mix that you want to aspirate and carefully reduce the squeezing pressure in order to create suction to aspirate the volume enough to reach the marketing. Then you move the pipette tip to the empty PCR tube and dispense the volume.**</caption> |
| </figure> | </figure> |
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