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pipettetip_thermocycling_imaging [2020/10/26 01:57]
richard |
pipettetip_thermocycling_imaging [2021/08/16 22:22] (current)
|
| M104 T1 S100 | M104 T1 S100 |
| </code> | </code> |
| | - Then you just assemble the macro steps to form the routine, it would look something like this for one cycle |
| | <code> |
| | //thermocycler_imaging |
| | //motion heatblock1 zh 0 row 1 F3000 |
| | G1Z0F3000 |
| | G1X34Y198.5Z0F3000_6 |
| | G1Z32F2000 |
| | G1Z42F400_30 |
| | //motion heatblock2 zh 0 row 1 F3000 |
| | G1Z0F3000 |
| | G1X36Y248Z0F3000_5 |
| | G1Z32F2000_2 |
| | G1Z42F400_180 |
| | G1Z0F3000 |
| | //motion PCRcam zh 0 row 1 F3000 |
| | G1Z0F3000 |
| | G1X5.5Y83.5F3000 |
| | G1Z46F3000 |
| | //camsnap 192.168.1.89_09302020070456_pipetteimaging 400 70 |
| | snap 192.168.1.89_400_70_09302020070456_pipetteimaging |
| | |
| | </code> |
| | |
| | |
| | This experiment tracks the volume per thermocycle (after 44 cycles). The aspiration conditions were as followed: |
| | - Air gap 6µl |
| | - Sample 6µl |
| | - Air gap 4µl |
| | - 94C 15 seconds |
| | - 60C 30 seconds |
| | |
| | |
| | | cycle 1 {{ :347_50_thcycl5124824newpic.png?200 | }} | cycle 2 {{ :347_50_thcycl6124936newpic.png?200 | }} | cycle 3 {{ :347_50_thcycl7125049newpic.png?200 | }} | cycle 4 {{ :347_50_thcycl8125202newpic.png?200 | }} | cycle 5 {{ :347_50_thcycl9125315newpic.png?200 | }} | |
| | | cycle 6 {{ :347_50_thcycl10125427newpic.png?200 | cycle 7 {{ :347_50_thcycl1124104newpic.png?200 | }} | cycle 8 {{ :347_50_thcycl12125653newpic.png?200 | }} | cycle 9 {{ :347_50_thcycl13125805newpic.png?200 | }} | cycle 10 {{ :347_50_thcycl14125918newpic.png?200 | }} | cycle 11 {{ :347_50_thcycl15130031newpic.png?200 | }} | |
| | | cycle 12 {{ :347_50_thcycl16130143newpic.png?200 | }} | cycle 13 {{ :347_50_thcycl17130256newpic.png?200 | }} | cycle 14 {{ :347_50_thcycl18130409newpic.png?200 | }} | cycle 15 {{ :347_50_thcycl19130522newpic.png?200 | }} | cycle 16 {{ :347_50_thcycl20130634newpic.png?200 | }} | |
| | | cycle 17 {{ :347_50_thcycl21130747newpic.png?200 | }} | cycle 18 {{ :347_50_thcycl2124229newpic.png?200 | }} | cycle 19 {{ :347_50_thcycl22130900newpic.png?200 | }} | cycle 20 {{ :347_50_thcycl23131012newpic.png?200 | }} | cycle 21 {{ :347_50_thcycl24131125newpic.png?200 | }} | |
| | | cycle 22 {{ :347_50_thcycl25131238newpic.png?200 | }} | cycle 23 {{ :347_50_thcycl26131350newpic.png?200 | }} | cycle 24 {{ :347_50_thcycl27131503newpic.png?200 | }} | cycle 25 {{ :347_50_thcycl28131616newpic.png?200 | }} | cycle 26 {{ :347_50_thcycl29131728newpic.png?200 | }} | |
| | | cycle 27 {{ :347_50_thcycl30131841newpic.png?200 | }} | cycle 28 {{ :347_50_thcycl31131954newpic.png?200 | }} | cycle 29 {{ :347_50_thcycl3124354newpic.png?200 | }} | cycle 30 {{ :347_50_thcycl32132107newpic.png?200 | }} | cycle 31 {{ :347_50_thcycl33132219newpic.png?200 | }} | |
| | | cycle 32 {{ :347_50_thcycl34132332newpic.png?200 | }} | cycle 33 {{ :347_50_thcycl35132445newpic.png?200 | }} | cycle 34 {{ :347_50_thcycl36132557newpic.png?200 | }} | cycle 35 {{ :347_50_thcycl37132710newpic.png?200 | }} | cycle 36 {{ :347_50_thcycl38132823newpic.png?200 | }} | |
| | | cycle 37 {{ :347_50_thcycl39132936newpic.png?200 | }} | cycle 38 {{ :347_50_thcycl40133048newpic.png?200 | }} | cycle 39 {{ :347_50_thcycl41133201newpic.png?200 | }} | cycle 40 {{ :347_50_thcycl4124519newpic.png?200 | }} | cycle 41 {{ :347_50_thcycl42133314newpic.png?200 | }} | |
| | | cycle 42 {{ :347_50_thcycl43133426newpic.png?200 | }} | cycle 43 {{ :347_50_thcycl44133539newpic.png?200 | }} | cycle 44 {{ :347_50_thcycl45133652newpic.png?200 | }} | |
| | |
| | |
| | ---- |
| | |
| | Mouse beta-actin PCR |
| | |
| | |
| | {{ :bdev:firstpipettepcrgraph.png?600 |}} |
| | |
| | |
| | |cycles|Template signal|Template image|No template signal|No template image| |
| | |1|86.93589743589743| {{ :bdev:template_350_60_thermocycle0pos56.5133827.png |}} |84.77777777777777| {{ :bdev:notemplate_350_60_thermocycle0pos54.5133806.png |}} | |
| | |2|88.48148148148148| {{ :bdev:template_350_60_thermocycle1pos58134036.png |}} |86.01754385964912| {{ :bdev:notemplate_350_60_thermocycle1pos54.5133959.png |}} | |
| | |3|89.98148148148148| {{ :bdev:template_350_60_thermocycle2pos58134229.png |}} |87.30952380952381| {{ :bdev:notemplate_350_60_thermocycle2pos58134229.png |}} | |
| | |4|87.81111111111112| {{ :bdev:template_350_60_thermocycle3pos56.5134407.png |}} |85.19047619047619| {{ :bdev:notemplate_350_60_thermocycle3pos56.5134407.png |}} | |
| | |5|91.03030303030303| {{ :bdev:template_350_60_thermocycle4pos57.5134610.png |}} |87.87719298245614| {{ :bdev:notemplate_350_60_thermocycle4pos57.5134610.png |}} | |
| | |6|91.22619047619048| {{ :bdev:template_350_60_thermocycle5pos56134748.png |}} |88.04545454545455| {{ :bdev:notemplate_350_60_thermocycle5pos57134759.png |}} | |
| | |7|89.44827586206897| {{ :bdev:template_350_60_thermocycle6pos56134941.png |}} |86.0| {{ :bdev:notemplate_350_60_thermocycle6pos53134910.png |}} | |
| | |8|90.93827160493827| {{ :bdev:template_350_60_thermocycle7pos56.5135140.png |}} |86.56666666666666| {{ :bdev:notemplate_350_60_thermocycle7pos54.5135119.png |}} | |
| | |9|88.73076923076923| {{ :bdev:template_350_60_thermocycle8pos56.5135333.png |}} |86.66666666666667| {{ :bdev:notemplate_350_60_thermocycle8pos58135349.png |}} | |
| | |10|92.92| {{ :bdev:template_350_60_thermocycle9pos57135532.png |}} |92.11111111111111| {{ :bdev:notemplate_350_60_thermocycle9pos54.5135506.png |}} | |
| | |11|88.4367816091954| {{ :bdev:template_350_60_thermocycle10pos54.5135705.png |}} |86.68253968253968| {{ :bdev:notemplate_350_60_thermocycle10pos56135721.png |}} | |
| | |12|87.72413793103448| {{ :bdev:template_350_60_thermocycle11pos55135904.png |}} |98.33333333333333| {{ :bdev:notemplate_350_60_thermocycle11pos58135935.png |}} | |
| | |13|88.18666666666667| {{ :bdev:template_350_60_thermocycle12pos56140108.png |}} |86.14035087719299| {{ :bdev:notemplate_350_60_thermocycle12pos56140108.png |}} | |
| | |14|90.09876543209876| {{ :bdev:template_350_60_thermocycle13pos55.5140256.png |}} |87.06410256410257| {{ :bdev:notemplate_350_60_thermocycle13pos55.5140256.png |}} | |
| | |15|88.0| {{ :bdev:template_350_60_thermocycle14pos55140444.png |}} |85.21794871794872| {{ :bdev:notemplate_350_60_thermocycle14pos55140444.png |}} | |
| | |16|88.46376811594203| {{ :bdev:template_350_60_thermocycle15pos56.5140653.png |}} |85.42222222222222| {{ :bdev:notemplate_350_60_thermocycle15pos56.5140653.png |}} | |
| | |17|89.38333333333334| {{ :bdev:template_350_60_thermocycle16pos57140851.png |}} |94.16666666666667| {{ :bdev:notemplate_350_60_thermocycle16pos58140902.png |}} | |
| | |18|93.52| {{ :bdev:template_350_60_thermocycle17pos55141024.png |}} |90.11111111111111| {{ :bdev:notemplate_350_60_thermocycle17pos55141024.png |}} | |
| | |19|92.88888888888889| {{ :bdev:template_350_60_thermocycle18pos55.5141222.png |}} |87.42666666666666| {{ :bdev:notemplate_350_60_thermocycle18pos55.5141222.png |}} | |
| | |20|91.51388888888889| {{ :bdev:template_350_60_thermocycle19pos55.5141416.png |}} |87.07575757575758| {{ :bdev:notemplate_350_60_thermocycle19pos55141410.png |}} | |
| | |21|93.17391304347827| {{ :bdev:template_350_60_thermocycle20pos55.5141615.png |}} |88.05797101449275| {{ :bdev:notemplate_350_60_thermocycle20pos55.5141615.png |}} | |
| | |22|93.81818181818181| {{ :bdev:template_350_60_thermocycle21pos56141814.png |}} |88.73015873015873| {{ :bdev:notemplate_350_60_thermocycle21pos56141814.png |}} | |
| | |23|96.39393939393939| {{ :bdev:template_350_60_thermocycle22pos55.5142002.png |}} |91.60606060606061| {{ :bdev:notemplate_350_60_thermocycle22pos55141957.png |}} | |
| | |24|94.98412698412699| {{ :bdev:template_350_60_thermocycle23pos56.5142206.png |}} |86.88888888888889| {{ :bdev:notemplate_350_60_thermocycle23pos56.5142206.png |}} | |
| | |25|96.15| {{ :bdev:template_350_60_thermocycle24pos56.5142359.png |}} |88.26984126984127| {{ :bdev:notemplate_350_60_thermocycle24pos55.5142348.png |}} | |
| | |26|96.2| {{ :bdev:template_350_60_thermocycle25pos56142547.png |}} |88.41666666666667| {{ :bdev:notemplate_350_60_thermocycle25pos56142547.png |}} | |
| | |27|97.0701754385965| {{ :bdev:template_350_60_thermocycle26pos56.5142745.png |}} |90.63333333333334| {{ :bdev:notemplate_350_60_thermocycle26pos54.5142725.png |}} | |
| | |28|98.14035087719299| {{ :bdev:template_350_60_thermocycle27pos56142934.png |}} |90.54385964912281| {{ :bdev:notemplate_350_60_thermocycle27pos56142934.png |}} | |
| | |29|98.01754385964912| {{ :bdev:template_350_60_thermocycle28pos57143137.png |}} |88.28070175438596| {{ :bdev:notemplate_350_60_thermocycle28pos55.5143122.png |}} | |
| | |30|97.79629629629629| {{ :bdev:template_350_60_thermocycle29pos56143320.png |}} |91.43859649122807| {{ :bdev:notemplate_350_60_thermocycle29pos55.5143315.png |}} | |
| | |31|103.03703703703704| {{ :bdev:template_350_60_thermocycle30pos57.5143859.png |}} |91.01960784313725| {{ :bdev:notemplate_350_60_thermocycle30pos56143844.png |}} | |
| | |32|100.3125| {{ :bdev:template_350_60_thermocycle31pos56.5144042.png |}} |91.80392156862744| {{ :bdev:notemplate_350_60_thermocycle31pos56144037.png |}} | |
| | |33|103.04166666666667| {{ :bdev:template_350_60_thermocycle32pos57144241.png |}} |92.3529411764706| {{ :bdev:notemplate_350_60_thermocycle32pos56144230.png |}} | |
| | |34|103.14583333333333| {{ :bdev:template_350_60_thermocycle33pos57144434.png |}} |93.125| {{ :bdev:notemplate_350_60_thermocycle33pos55.5144418.png |}} | |
| | |35|105.8| {{ :bdev:template_350_60_thermocycle34pos57.5144633.png |}} |93.4| {{ :bdev:notemplate_350_60_thermocycle34pos55.5144612.png |}} | |
| | |36|104.9375| {{ :bdev:template_350_60_thermocycle35pos57.5144826.png |}} |95.53333333333333| {{ :bdev:notemplate_350_60_thermocycle35pos56144810.png |}} | |
| | |37|109.14285714285714| {{ :bdev:template_350_60_thermocycle36pos57145014.png |}} |95.24444444444444| {{ :bdev:notemplate_350_60_thermocycle36pos55144953.png |}} | |
| | |38|104.85714285714286| {{ :bdev:template_350_60_thermocycle37pos56145157.png |}} |97.97619047619048| {{ :bdev:notemplate_350_60_thermocycle37pos56145157.png |}} | |
| | |39|109.78571428571429| {{ :bdev:template_350_60_thermocycle38pos57.5145406.png |}} |95.30952380952381| {{ :bdev:notemplate_350_60_thermocycle38pos55.5145345.png |}} | |
| | |40|108.51282051282051| {{ :bdev:template_350_60_thermocycle39pos57.5145559.png |}} |96.5| {{ :bdev:notemplate_350_60_thermocycle39pos55.5145538.png |}} | |
| | |41|112.7948717948718| {{ :bdev:template_350_60_thermocycle40pos57145747.png |}} |98.5| {{ :bdev:notemplate_350_60_thermocycle40pos56150118.png |}} | |
| | |42|116.57575757575758| {{ :bdev:template_350_60_thermocycle41pos57.5150327.png |}} |102.02777777777777| {{ :bdev:notemplate_350_60_thermocycle41pos56150312.png |}} | |
| | |43|114.57575757575758| {{ :bdev:template_350_60_thermocycle42pos57150516.png |}} |99.11111111111111| {{ :bdev:notemplate_350_60_thermocycle42pos56.5150510.png |}} | |
| | |44|116.87878787878788| {{ :bdev:template_350_60_thermocycle43pos57.5150714.png |}} |102.21212121212122| {{ :bdev:notemplate_350_60_thermocycle43pos55.5150653.png |}} | |
| | |45|119.06060606060606| {{ :bdev:template_350_60_thermocycle44pos57.5150907.png |}} |100.42424242424242| {{ :bdev:notemplate_350_60_thermocycle44pos55150841.png |}} | |
| | |46|119.93333333333334| {{ :bdev:template_350_60_thermocycle45pos57.5151101.png |}} |101.15151515151516| {{ :bdev:notemplate_350_60_thermocycle45pos55.5151040.png |}} | |
| | |47|131.53333333333333| {{ :bdev:template_350_60_thermocycle46pos57.5151254.png |}} |102.45454545454545| {{ :bdev:notemplate_350_60_thermocycle46pos57151249.png |}} | |
| | |48|123.23333333333333| {{ :bdev:template_350_60_thermocycle47pos58151452.png |}} |106.33333333333333| {{ :bdev:notemplate_350_60_thermocycle47pos56151432.png |}} | |
| | |49|123.39393939393939| {{ :bdev:template_350_60_thermocycle48pos58151646.png |}} |106.9| {{ :bdev:notemplate_350_60_thermocycle48pos56151625.png |}} | |
| | |50|124.66666666666667| {{ :bdev:template_350_60_thermocycle49pos57.5151834.png |}} |111.26666666666667| {{ :bdev:notemplate_350_60_thermocycle49pos55.5151813.png |}} | |
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