labautobox

User Tools

Site Tools

Trace:
pipettetip_thermocycling_imaging

Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1164

Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1168

Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1171

Warning: Trying to access array offset on value of type bool in /var/www/html/dokuwiki/inc/html.php on line 1172

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision 		Previous revision
pipettetip_thermocycling_imaging [2020/11/07 02:51]
richard 		pipettetip_thermocycling_imaging [2021/08/16 22:22] (current)
Line 37: Line 37:
  
  
-| cycle 1 {{ :347_50_afteraspiration123940newpic.png?200 | }} | cycle 2 {{ :347_50_thcycl10125427newpic.png?200 | }} | cycle 3 {{ :347_50_thcycl11125540newpic.png?200 | }} | cycle 4 {{ :347_50_thcycl1124104newpic.png?200 | }} | cycle 5 {{ :347_50_thcycl12125653newpic.png?200 | }} | cycle 6 {{ :347_50_thcycl13125805newpic.png?200 | }} | +This experiment tracks the volume per thermocycle (after 44 cycles). The aspiration conditions were as followed: 
-| cycle 7 {{ :347_50_thcycl14125918newpic.png?200 | }} | cycle 8 {{ :347_50_thcycl15130031newpic.png?200 | }} | cycle 9 {{ :347_50_thcycl16130143newpic.png?200 | }} | cycle 10 {{ :347_50_thcycl17130256newpic.png?200 | }} | cycle 11 {{ :347_50_thcycl18130409newpic.png?200 | }} | cycle 12 {{ :347_50_thcycl19130522newpic.png?200 | }} | +  - Air gap 6µl 
-| cycle 13 {{ :347_50_thcycl20130634newpic.png?200 | }} | cycle 14 {{ :347_50_thcycl21130747newpic.png?200 | }} | cycle 15 {{ :347_50_thcycl2124229newpic.png?200 | }} | cycle 16 {{ :347_50_thcycl22130900newpic.png?200 | }} | cycle 17 {{ :347_50_thcycl23131012newpic.png?200 | }} | cycle 18 {{ :347_50_thcycl24131125newpic.png?200 | }} | +  - Sample 6µl 
-| cycle 19 {{ :347_50_thcycl25131238newpic.png?200 | }} | cycle 20 {{ :347_50_thcycl26131350newpic.png?200 | }} | cycle 21 {{ :347_50_thcycl27131503newpic.png?200 | }} | cycle 22 {{ :347_50_thcycl28131616newpic.png?200 | }} | cycle 23 {{ :347_50_thcycl29131728newpic.png?200 | }} | cycle 24 {{ :347_50_thcycl30131841newpic.png?200 | }} | +  - Air gap 4µl 
-| cycle 25 {{ :347_50_thcycl31131954newpic.png?200 | }} | cycle 26 {{ :347_50_thcycl3124354newpic.png?200 | }} | cycle 27 {{ :347_50_thcycl32132107newpic.png?200 | }} | cycle 28 {{ :347_50_thcycl33132219newpic.png?200 | }} | cycle 29 {{ :347_50_thcycl34132332newpic.png?200 | }} | cycle 30 {{ :347_50_thcycl35132445newpic.png?200 | }} | +  - 94C 15 seconds 
-| cycle 31 {{ :347_50_thcycl36132557newpic.png?200 | }} | cycle 32 {{ :347_50_thcycl37132710newpic.png?200 | }} | cycle 33 {{ :347_50_thcycl38132823newpic.png?200 | }} | cycle 34 {{ :347_50_thcycl39132936newpic.png?200 | }} | cycle 35 {{ :347_50_thcycl40133048newpic.png?200 | }} | cycle 36 {{ :347_50_thcycl41133201newpic.png?200 | }} | +  - 60C 30 seconds 
-| cycle 37 {{ :347_50_thcycl4124519newpic.png?200 | }} | cycle 38 {{ :347_50_thcycl42133314newpic.png?200 | }} | cycle 39 {{ :347_50_thcycl43133426newpic.png?200 | }} | cycle 40 {{ :347_50_thcycl44133539newpic.png?200 | }} | cycle 41 {{ :347_50_thcycl45133652newpic.png?200 | }} | cycle 42 {{ :347_50_thcycl5124643newpic.png?200 | }} | + 
-| cycle 43 {{ :347_50_thcycl5124824newpic.png?200 | }} | cycle 44 {{ :347_50_thcycl6124936newpic.png?200 | }} | cycle 45 {{ :347_50_thcycl7125049newpic.png?200 | }} | cycle 46 {{ :347_50_thcycl8125202newpic.png?200 | }} | cycle 47 {{ :347_50_thcycl9125315newpic.png?200 | }} |+ 
 +| cycle 1 {{ :347_50_thcycl5124824newpic.png?200 | }} | cycle 2 {{ :347_50_thcycl6124936newpic.png?200 | }} | cycle 3 {{ :347_50_thcycl7125049newpic.png?200 | }} | cycle 4 {{ :347_50_thcycl8125202newpic.png?200 | }} | cycle 5 {{ :347_50_thcycl9125315newpic.png?200 | }} | 
 +| cycle 6 {{ :347_50_thcycl10125427newpic.png?200 | cycle 7 {{ :347_50_thcycl1124104newpic.png?200 | }} | cycle 8 {{ :347_50_thcycl12125653newpic.png?200 | }} | cycle 9 {{ :347_50_thcycl13125805newpic.png?200 | }} | cycle 10 {{ :347_50_thcycl14125918newpic.png?200 | }} | cycle 11 {{ :347_50_thcycl15130031newpic.png?200 | }} | 
 +| cycle 12 {{ :347_50_thcycl16130143newpic.png?200 | }} | cycle 13 {{ :347_50_thcycl17130256newpic.png?200 | }} | cycle 14 {{ :347_50_thcycl18130409newpic.png?200 | }} | cycle 15 {{ :347_50_thcycl19130522newpic.png?200 | }} | cycle 16 {{ :347_50_thcycl20130634newpic.png?200 | }} | 
 +| cycle 17 {{ :347_50_thcycl21130747newpic.png?200 | }} | cycle 18 {{ :347_50_thcycl2124229newpic.png?200 | }} | cycle 19 {{ :347_50_thcycl22130900newpic.png?200 | }} | cycle 20 {{ :347_50_thcycl23131012newpic.png?200 | }} | cycle 21 {{ :347_50_thcycl24131125newpic.png?200 | }} | 
 +| cycle 22 {{ :347_50_thcycl25131238newpic.png?200 | }} | cycle 23 {{ :347_50_thcycl26131350newpic.png?200 | }} | cycle 24 {{ :347_50_thcycl27131503newpic.png?200 | }} | cycle 25 {{ :347_50_thcycl28131616newpic.png?200 | }} | cycle 26 {{ :347_50_thcycl29131728newpic.png?200 | }} | 
 +| cycle 27 {{ :347_50_thcycl30131841newpic.png?200 | }} | cycle 28 {{ :347_50_thcycl31131954newpic.png?200 | }} | cycle 29 {{ :347_50_thcycl3124354newpic.png?200 | }} | cycle 30 {{ :347_50_thcycl32132107newpic.png?200 | }} | cycle 31 {{ :347_50_thcycl33132219newpic.png?200 | }} | 
 +| cycle 32 {{ :347_50_thcycl34132332newpic.png?200 | }} | cycle 33 {{ :347_50_thcycl35132445newpic.png?200 | }} | cycle 34 {{ :347_50_thcycl36132557newpic.png?200 | }} | cycle 35 {{ :347_50_thcycl37132710newpic.png?200 | }} | cycle 36 {{ :347_50_thcycl38132823newpic.png?200 | }} | 
 +| cycle 37 {{ :347_50_thcycl39132936newpic.png?200 | }} | cycle 38 {{ :347_50_thcycl40133048newpic.png?200 | }} | cycle 39 {{ :347_50_thcycl41133201newpic.png?200 | }} | cycle 40 {{ :347_50_thcycl4124519newpic.png?200 | }} | cycle 41 {{ :347_50_thcycl42133314newpic.png?200 | }} | 
 +| cycle 42 {{ :347_50_thcycl43133426newpic.png?200 | }} | cycle 43 {{ :347_50_thcycl44133539newpic.png?200 | }} | cycle 44 {{ :347_50_thcycl45133652newpic.png?200 | }} | 
 + 
 + 
 +---- 
 + 
 +Mouse beta-actin PCR 
 + 
 + 
 +{{ :bdev:firstpipettepcrgraph.png?600 |}} 
 + 
 + 
 +|cycles|Template signal|Template image|No template signal|No template image| 
 +|1|86.93589743589743| {{ :bdev:template_350_60_thermocycle0pos56.5133827.png |}} |84.77777777777777| {{ :bdev:notemplate_350_60_thermocycle0pos54.5133806.png |}} | 
 +|2|88.48148148148148| {{ :bdev:template_350_60_thermocycle1pos58134036.png |}} |86.01754385964912| {{ :bdev:notemplate_350_60_thermocycle1pos54.5133959.png |}} | 
 +|3|89.98148148148148| {{ :bdev:template_350_60_thermocycle2pos58134229.png |}} |87.30952380952381| {{ :bdev:notemplate_350_60_thermocycle2pos58134229.png |}} | 
 +|4|87.81111111111112| {{ :bdev:template_350_60_thermocycle3pos56.5134407.png |}} |85.19047619047619| {{ :bdev:notemplate_350_60_thermocycle3pos56.5134407.png |}} | 
 +|5|91.03030303030303| {{ :bdev:template_350_60_thermocycle4pos57.5134610.png |}} |87.87719298245614| {{ :bdev:notemplate_350_60_thermocycle4pos57.5134610.png |}} | 
 +|6|91.22619047619048| {{ :bdev:template_350_60_thermocycle5pos56134748.png |}} |88.04545454545455| {{ :bdev:notemplate_350_60_thermocycle5pos57134759.png |}} | 
 +|7|89.44827586206897| {{ :bdev:template_350_60_thermocycle6pos56134941.png |}} |86.0| {{ :bdev:notemplate_350_60_thermocycle6pos53134910.png |}} | 
 +|8|90.93827160493827| {{ :bdev:template_350_60_thermocycle7pos56.5135140.png |}} |86.56666666666666| {{ :bdev:notemplate_350_60_thermocycle7pos54.5135119.png |}} | 
 +|9|88.73076923076923| {{ :bdev:template_350_60_thermocycle8pos56.5135333.png |}} |86.66666666666667| {{ :bdev:notemplate_350_60_thermocycle8pos58135349.png |}} | 
 +|10|92.92| {{ :bdev:template_350_60_thermocycle9pos57135532.png |}} |92.11111111111111| {{ :bdev:notemplate_350_60_thermocycle9pos54.5135506.png |}} | 
 +|11|88.4367816091954| {{ :bdev:template_350_60_thermocycle10pos54.5135705.png |}} |86.68253968253968| {{ :bdev:notemplate_350_60_thermocycle10pos56135721.png |}} | 
 +|12|87.72413793103448| {{ :bdev:template_350_60_thermocycle11pos55135904.png |}} |98.33333333333333| {{ :bdev:notemplate_350_60_thermocycle11pos58135935.png |}} | 
 +|13|88.18666666666667| {{ :bdev:template_350_60_thermocycle12pos56140108.png |}} |86.14035087719299| {{ :bdev:notemplate_350_60_thermocycle12pos56140108.png |}} | 
 +|14|90.09876543209876| {{ :bdev:template_350_60_thermocycle13pos55.5140256.png |}} |87.06410256410257| {{ :bdev:notemplate_350_60_thermocycle13pos55.5140256.png |}} | 
 +|15|88.0| {{ :bdev:template_350_60_thermocycle14pos55140444.png |}} |85.21794871794872| {{ :bdev:notemplate_350_60_thermocycle14pos55140444.png |}} | 
 +|16|88.46376811594203| {{ :bdev:template_350_60_thermocycle15pos56.5140653.png |}} |85.42222222222222| {{ :bdev:notemplate_350_60_thermocycle15pos56.5140653.png |}} | 
 +|17|89.38333333333334| {{ :bdev:template_350_60_thermocycle16pos57140851.png |}} |94.16666666666667| {{ :bdev:notemplate_350_60_thermocycle16pos58140902.png |}} | 
 +|18|93.52| {{ :bdev:template_350_60_thermocycle17pos55141024.png |}} |90.11111111111111| {{ :bdev:notemplate_350_60_thermocycle17pos55141024.png |}} | 
 +|19|92.88888888888889| {{ :bdev:template_350_60_thermocycle18pos55.5141222.png |}} |87.42666666666666| {{ :bdev:notemplate_350_60_thermocycle18pos55.5141222.png |}} | 
 +|20|91.51388888888889| {{ :bdev:template_350_60_thermocycle19pos55.5141416.png |}} |87.07575757575758| {{ :bdev:notemplate_350_60_thermocycle19pos55141410.png |}} | 
 +|21|93.17391304347827| {{ :bdev:template_350_60_thermocycle20pos55.5141615.png |}} |88.05797101449275| {{ :bdev:notemplate_350_60_thermocycle20pos55.5141615.png |}} | 
 +|22|93.81818181818181| {{ :bdev:template_350_60_thermocycle21pos56141814.png |}} |88.73015873015873| {{ :bdev:notemplate_350_60_thermocycle21pos56141814.png |}} | 
 +|23|96.39393939393939| {{ :bdev:template_350_60_thermocycle22pos55.5142002.png |}} |91.60606060606061| {{ :bdev:notemplate_350_60_thermocycle22pos55141957.png |}} | 
 +|24|94.98412698412699| {{ :bdev:template_350_60_thermocycle23pos56.5142206.png |}} |86.88888888888889| {{ :bdev:notemplate_350_60_thermocycle23pos56.5142206.png |}} | 
 +|25|96.15| {{ :bdev:template_350_60_thermocycle24pos56.5142359.png |}} |88.26984126984127| {{ :bdev:notemplate_350_60_thermocycle24pos55.5142348.png |}} | 
 +|26|96.2| {{ :bdev:template_350_60_thermocycle25pos56142547.png |}} |88.41666666666667| {{ :bdev:notemplate_350_60_thermocycle25pos56142547.png |}} | 
 +|27|97.0701754385965| {{ :bdev:template_350_60_thermocycle26pos56.5142745.png |}} |90.63333333333334| {{ :bdev:notemplate_350_60_thermocycle26pos54.5142725.png |}} | 
 +|28|98.14035087719299| {{ :bdev:template_350_60_thermocycle27pos56142934.png |}} |90.54385964912281| {{ :bdev:notemplate_350_60_thermocycle27pos56142934.png |}} | 
 +|29|98.01754385964912| {{ :bdev:template_350_60_thermocycle28pos57143137.png |}} |88.28070175438596| {{ :bdev:notemplate_350_60_thermocycle28pos55.5143122.png |}} | 
 +|30|97.79629629629629| {{ :bdev:template_350_60_thermocycle29pos56143320.png |}} |91.43859649122807| {{ :bdev:notemplate_350_60_thermocycle29pos55.5143315.png |}} | 
 +|31|103.03703703703704| {{ :bdev:template_350_60_thermocycle30pos57.5143859.png |}} |91.01960784313725| {{ :bdev:notemplate_350_60_thermocycle30pos56143844.png |}} | 
 +|32|100.3125| {{ :bdev:template_350_60_thermocycle31pos56.5144042.png |}} |91.80392156862744| {{ :bdev:notemplate_350_60_thermocycle31pos56144037.png |}} | 
 +|33|103.04166666666667| {{ :bdev:template_350_60_thermocycle32pos57144241.png |}} |92.3529411764706| {{ :bdev:notemplate_350_60_thermocycle32pos56144230.png |}} | 
 +|34|103.14583333333333| {{ :bdev:template_350_60_thermocycle33pos57144434.png |}} |93.125| {{ :bdev:notemplate_350_60_thermocycle33pos55.5144418.png |}} | 
 +|35|105.8| {{ :bdev:template_350_60_thermocycle34pos57.5144633.png |}} |93.4| {{ :bdev:notemplate_350_60_thermocycle34pos55.5144612.png |}} | 
 +|36|104.9375| {{ :bdev:template_350_60_thermocycle35pos57.5144826.png |}} |95.53333333333333| {{ :bdev:notemplate_350_60_thermocycle35pos56144810.png |}} | 
 +|37|109.14285714285714| {{ :bdev:template_350_60_thermocycle36pos57145014.png |}} |95.24444444444444| {{ :bdev:notemplate_350_60_thermocycle36pos55144953.png |}} | 
 +|38|104.85714285714286| {{ :bdev:template_350_60_thermocycle37pos56145157.png |}} |97.97619047619048| {{ :bdev:notemplate_350_60_thermocycle37pos56145157.png |}} | 
 +|39|109.78571428571429| {{ :bdev:template_350_60_thermocycle38pos57.5145406.png |}} |95.30952380952381| {{ :bdev:notemplate_350_60_thermocycle38pos55.5145345.png |}} | 
 +|40|108.51282051282051| {{ :bdev:template_350_60_thermocycle39pos57.5145559.png |}} |96.5| {{ :bdev:notemplate_350_60_thermocycle39pos55.5145538.png |}} | 
 +|41|112.7948717948718| {{ :bdev:template_350_60_thermocycle40pos57145747.png |}} |98.5| {{ :bdev:notemplate_350_60_thermocycle40pos56150118.png |}} | 
 +|42|116.57575757575758| {{ :bdev:template_350_60_thermocycle41pos57.5150327.png |}} |102.02777777777777| {{ :bdev:notemplate_350_60_thermocycle41pos56150312.png |}} | 
 +|43|114.57575757575758| {{ :bdev:template_350_60_thermocycle42pos57150516.png |}} |99.11111111111111| {{ :bdev:notemplate_350_60_thermocycle42pos56.5150510.png |}} | 
 +|44|116.87878787878788| {{ :bdev:template_350_60_thermocycle43pos57.5150714.png |}} |102.21212121212122| {{ :bdev:notemplate_350_60_thermocycle43pos55.5150653.png |}} | 
 +|45|119.06060606060606| {{ :bdev:template_350_60_thermocycle44pos57.5150907.png |}} |100.42424242424242| {{ :bdev:notemplate_350_60_thermocycle44pos55150841.png |}} | 
 +|46|119.93333333333334| {{ :bdev:template_350_60_thermocycle45pos57.5151101.png |}} |101.15151515151516| {{ :bdev:notemplate_350_60_thermocycle45pos55.5151040.png |}} | 
 +|47|131.53333333333333| {{ :bdev:template_350_60_thermocycle46pos57.5151254.png |}} |102.45454545454545| {{ :bdev:notemplate_350_60_thermocycle46pos57151249.png |}} | 
 +|48|123.23333333333333| {{ :bdev:template_350_60_thermocycle47pos58151452.png |}} |106.33333333333333| {{ :bdev:notemplate_350_60_thermocycle47pos56151432.png |}} | 
 +|49|123.39393939393939| {{ :bdev:template_350_60_thermocycle48pos58151646.png |}} |106.9| {{ :bdev:notemplate_350_60_thermocycle48pos56151625.png |}} | 
 +|50|124.66666666666667| {{ :bdev:template_350_60_thermocycle49pos57.5151834.png |}} |111.26666666666667| {{ :bdev:notemplate_350_60_thermocycle49pos55.5151813.png |}} | 
 + 
pipettetip_thermocycling_imaging.1604717506.txt.gz · Last modified: 2021/08/16 22:21 (external edit)

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: GNU Free Documentation License 1.3
GNU Free Documentation License 1.3 Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki