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pipettetip_thermocycling_imaging

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pipettetip_thermocycling_imaging [2020/11/07 03:17]
richard 		pipettetip_thermocycling_imaging [2021/08/16 22:22] (current)
Line 37: Line 37:
  
  
-This experiment tracks the volume per thermocycle (after 45 cycles). The aspiration conditions were as followed:+This experiment tracks the volume per thermocycle (after 44 cycles). The aspiration conditions were as followed:
   - Air gap 6µl   - Air gap 6µl
   - Sample 6µl   - Sample 6µl
Line 53: Line 53:
 | cycle 32 {{ :347_50_thcycl34132332newpic.png?200 | }} | cycle 33 {{ :347_50_thcycl35132445newpic.png?200 | }} | cycle 34 {{ :347_50_thcycl36132557newpic.png?200 | }} | cycle 35 {{ :347_50_thcycl37132710newpic.png?200 | }} | cycle 36 {{ :347_50_thcycl38132823newpic.png?200 | }} | | cycle 32 {{ :347_50_thcycl34132332newpic.png?200 | }} | cycle 33 {{ :347_50_thcycl35132445newpic.png?200 | }} | cycle 34 {{ :347_50_thcycl36132557newpic.png?200 | }} | cycle 35 {{ :347_50_thcycl37132710newpic.png?200 | }} | cycle 36 {{ :347_50_thcycl38132823newpic.png?200 | }} |
 | cycle 37 {{ :347_50_thcycl39132936newpic.png?200 | }} | cycle 38 {{ :347_50_thcycl40133048newpic.png?200 | }} | cycle 39 {{ :347_50_thcycl41133201newpic.png?200 | }} | cycle 40 {{ :347_50_thcycl4124519newpic.png?200 | }} | cycle 41 {{ :347_50_thcycl42133314newpic.png?200 | }} | | cycle 37 {{ :347_50_thcycl39132936newpic.png?200 | }} | cycle 38 {{ :347_50_thcycl40133048newpic.png?200 | }} | cycle 39 {{ :347_50_thcycl41133201newpic.png?200 | }} | cycle 40 {{ :347_50_thcycl4124519newpic.png?200 | }} | cycle 41 {{ :347_50_thcycl42133314newpic.png?200 | }} |
-| cycle 42 {{ :347_50_thcycl43133426newpic.png?200 | }} | cycle 43 {{ :347_50_thcycl44133539newpic.png?200 | }} | cycle 44 {{ :347_50_thcycl45133652newpic.png?200 | }} | cycle 45 {{ :347_50_thcycl5124643newpic.png?200 | }} |+| cycle 42 {{ :347_50_thcycl43133426newpic.png?200 | }} | cycle 43 {{ :347_50_thcycl44133539newpic.png?200 | }} | cycle 44 {{ :347_50_thcycl45133652newpic.png?200 | }} |
  
  
 +----
  
-The LabBot macro:+Mouse beta-actin PCR
  
  
-<code> +{{ :bdev:firstpipettepcrgraph.png?600 |}} 
-//45cycle + 
-//aspthermocycle12 + 
-//shakevalves +|cycles|Template signal|Template image|No template signal|No template image| 
-//valve-1_1_1_1_1_1_1_1-valveinput +|1|86.93589743589743| {{ :bdev:template_350_60_thermocycle0pos56.5133827.png |}} |84.77777777777777| {{ :bdev:notemplate_350_60_thermocycle0pos54.5133806.png |}} | 
-valve-1_1_1_1_1_1_1_1-input +|2|88.48148148148148| {{ :bdev:template_350_60_thermocycle1pos58134036.png |}} |86.01754385964912| {{ :bdev:notemplate_350_60_thermocycle1pos54.5133959.png |}} | 
-G1Z0F1000_0.5 +|3|89.98148148148148| {{ :bdev:template_350_60_thermocycle2pos58134229.png |}} |87.30952380952381| {{ :bdev:notemplate_350_60_thermocycle2pos58134229.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveoutput +|4|87.81111111111112| {{ :bdev:template_350_60_thermocycle3pos56.5134407.png |}} |85.19047619047619| {{ :bdev:notemplate_350_60_thermocycle3pos56.5134407.png |}} | 
-valve-1_1_1_1_1_1_1_1-output +|5|91.03030303030303| {{ :bdev:template_350_60_thermocycle4pos57.5134610.png |}} |87.87719298245614| {{ :bdev:notemplate_350_60_thermocycle4pos57.5134610.png |}} | 
-G1Z0F1000_0.5 +|6|91.22619047619048| {{ :bdev:template_350_60_thermocycle5pos56134748.png |}} |88.04545454545455| {{ :bdev:notemplate_350_60_thermocycle5pos57134759.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveinput +|7|89.44827586206897| {{ :bdev:template_350_60_thermocycle6pos56134941.png |}} |86.0| {{ :bdev:notemplate_350_60_thermocycle6pos53134910.png |}} | 
-valve-1_1_1_1_1_1_1_1-input +|8|90.93827160493827| {{ :bdev:template_350_60_thermocycle7pos56.5135140.png |}} |86.56666666666666| {{ :bdev:notemplate_350_60_thermocycle7pos54.5135119.png |}} | 
-G1Z0F1000_0.5 +|9|88.73076923076923| {{ :bdev:template_350_60_thermocycle8pos56.5135333.png |}} |86.66666666666667| {{ :bdev:notemplate_350_60_thermocycle8pos58135349.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveoutput +|10|92.92| {{ :bdev:template_350_60_thermocycle9pos57135532.png |}} |92.11111111111111| {{ :bdev:notemplate_350_60_thermocycle9pos54.5135506.png |}} | 
-valve-1_1_1_1_1_1_1_1-output +|11|88.4367816091954| {{ :bdev:template_350_60_thermocycle10pos54.5135705.png |}} |86.68253968253968| {{ :bdev:notemplate_350_60_thermocycle10pos56135721.png |}} | 
-G1Z0F1000_0.5 +|12|87.72413793103448| {{ :bdev:template_350_60_thermocycle11pos55135904.png |}} |98.33333333333333| {{ :bdev:notemplate_350_60_thermocycle11pos58135935.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveinput +|13|88.18666666666667| {{ :bdev:template_350_60_thermocycle12pos56140108.png |}} |86.14035087719299| {{ :bdev:notemplate_350_60_thermocycle12pos56140108.png |}} | 
-valve-1_1_1_1_1_1_1_1-input +|14|90.09876543209876| {{ :bdev:template_350_60_thermocycle13pos55.5140256.png |}} |87.06410256410257| {{ :bdev:notemplate_350_60_thermocycle13pos55.5140256.png |}} | 
-G1Z0F1000_0.5 +|15|88.0| {{ :bdev:template_350_60_thermocycle14pos55140444.png |}} |85.21794871794872| {{ :bdev:notemplate_350_60_thermocycle14pos55140444.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveoutput +|16|88.46376811594203| {{ :bdev:template_350_60_thermocycle15pos56.5140653.png |}} |85.42222222222222| {{ :bdev:notemplate_350_60_thermocycle15pos56.5140653.png |}} | 
-valve-1_1_1_1_1_1_1_1-output +|17|89.38333333333334| {{ :bdev:template_350_60_thermocycle16pos57140851.png |}} |94.16666666666667| {{ :bdev:notemplate_350_60_thermocycle16pos58140902.png |}} | 
-G1Z0F1000_0.5 +|18|93.52| {{ :bdev:template_350_60_thermocycle17pos55141024.png |}} |90.11111111111111| {{ :bdev:notemplate_350_60_thermocycle17pos55141024.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveinput +|19|92.88888888888889| {{ :bdev:template_350_60_thermocycle18pos55.5141222.png |}} |87.42666666666666| {{ :bdev:notemplate_350_60_thermocycle18pos55.5141222.png |}} | 
-valve-1_1_1_1_1_1_1_1-input +|20|91.51388888888889| {{ :bdev:template_350_60_thermocycle19pos55.5141416.png |}} |87.07575757575758| {{ :bdev:notemplate_350_60_thermocycle19pos55141410.png |}} | 
-//syringe 300 speed 40 acc 20 +|21|93.17391304347827| {{ :bdev:template_350_60_thermocycle20pos55.5141615.png |}} |88.05797101449275| {{ :bdev:notemplate_350_60_thermocycle20pos55.5141615.png |}} | 
-sg1e300s40a20_20 +|22|93.81818181818181| {{ :bdev:template_350_60_thermocycle21pos56141814.png |}} |88.73015873015873| {{ :bdev:notemplate_350_60_thermocycle21pos56141814.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveoutput +|23|96.39393939393939| {{ :bdev:template_350_60_thermocycle22pos55.5142002.png |}} |91.60606060606061| {{ :bdev:notemplate_350_60_thermocycle22pos55141957.png |}} | 
-valve-1_1_1_1_1_1_1_1-output +|24|94.98412698412699| {{ :bdev:template_350_60_thermocycle23pos56.5142206.png |}} |86.88888888888889| {{ :bdev:notemplate_350_60_thermocycle23pos56.5142206.png |}} | 
-sg1e306s6a3_6 +|25|96.15| {{ :bdev:template_350_60_thermocycle24pos56.5142359.png |}} |88.26984126984127| {{ :bdev:notemplate_350_60_thermocycle24pos55.5142348.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valvebypass +|26|96.2| {{ :bdev:template_350_60_thermocycle25pos56142547.png |}} |88.41666666666667| {{ :bdev:notemplate_350_60_thermocycle25pos56142547.png |}} | 
-valve-1_1_1_1_1_1_1_1-bypass +|27|97.0701754385965| {{ :bdev:template_350_60_thermocycle26pos56.5142745.png |}} |90.63333333333334| {{ :bdev:notemplate_350_60_thermocycle26pos54.5142725.png |}} | 
-//dyeaspmodel +|28|98.14035087719299| {{ :bdev:template_350_60_thermocycle27pos56142934.png |}} |90.54385964912281| {{ :bdev:notemplate_350_60_thermocycle27pos56142934.png |}} | 
-//dyeaspmodel +|29|98.01754385964912| {{ :bdev:template_350_60_thermocycle28pos57143137.png |}} |88.28070175438596| {{ :bdev:notemplate_350_60_thermocycle28pos55.5143122.png |}} | 
-//motion 96well zh 20 row 2 F3000 +|30|97.79629629629629| {{ :bdev:template_350_60_thermocycle29pos56143320.png |}} |91.43859649122807| {{ :bdev:notemplate_350_60_thermocycle29pos55.5143315.png |}} | 
-G1Z0F3000 +|31|103.03703703703704| {{ :bdev:template_350_60_thermocycle30pos57.5143859.png |}} |91.01960784313725| {{ :bdev:notemplate_350_60_thermocycle30pos56143844.png |}} | 
-G1X228.16666666667Y183.5F5000_8 +|32|100.3125| {{ :bdev:template_350_60_thermocycle31pos56.5144042.png |}} |91.80392156862744| {{ :bdev:notemplate_350_60_thermocycle31pos56144037.png |}} | 
-G1Z40F3000 +|33|103.04166666666667| {{ :bdev:template_350_60_thermocycle32pos57144241.png |}} |92.3529411764706| {{ :bdev:notemplate_350_60_thermocycle32pos56144230.png |}} | 
-//motion 96well zh 0 row 2 F500 +|34|103.14583333333333| {{ :bdev:template_350_60_thermocycle33pos57144434.png |}} |93.125| {{ :bdev:notemplate_350_60_thermocycle33pos55.5144418.png |}} | 
-G1X228.16666666667Y183.5F500 +|35|105.8| {{ :bdev:template_350_60_thermocycle34pos57.5144633.png |}} |93.4| {{ :bdev:notemplate_350_60_thermocycle34pos55.5144612.png |}} | 
-G1Z60F500_5 +|36|104.9375| {{ :bdev:template_350_60_thermocycle35pos57.5144826.png |}} |95.53333333333333| {{ :bdev:notemplate_350_60_thermocycle35pos56144810.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveoutput +|37|109.14285714285714| {{ :bdev:template_350_60_thermocycle36pos57145014.png |}} |95.24444444444444| {{ :bdev:notemplate_350_60_thermocycle36pos55144953.png |}} | 
-valve-1_1_1_1_1_1_1_1-output +|38|104.85714285714286| {{ :bdev:template_350_60_thermocycle37pos56145157.png |}} |97.97619047619048| {{ :bdev:notemplate_350_60_thermocycle37pos56145157.png |}} | 
-//syringe 326 speed 6 acc 3 +|39|109.78571428571429| {{ :bdev:template_350_60_thermocycle38pos57.5145406.png |}} |95.30952380952381| {{ :bdev:notemplate_350_60_thermocycle38pos55.5145345.png |}} | 
-sg1e312s6a3_8 +|40|108.51282051282051| {{ :bdev:template_350_60_thermocycle39pos57.5145559.png |}} |96.5| {{ :bdev:notemplate_350_60_thermocycle39pos55.5145538.png |}} | 
-G1Z60F500_2 +|41|112.7948717948718| {{ :bdev:template_350_60_thermocycle40pos57145747.png |}} |98.5| {{ :bdev:notemplate_350_60_thermocycle40pos56150118.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valvebypass +|42|116.57575757575758| {{ :bdev:template_350_60_thermocycle41pos57.5150327.png |}} |102.02777777777777| {{ :bdev:notemplate_350_60_thermocycle41pos56150312.png |}} | 
-valve-1_1_1_1_1_1_1_1-bypass +|43|114.57575757575758| {{ :bdev:template_350_60_thermocycle42pos57150516.png |}} |99.11111111111111| {{ :bdev:notemplate_350_60_thermocycle42pos56.5150510.png |}} | 
-//motion 96well zh 20 row 2 F3000 +|44|116.87878787878788| {{ :bdev:template_350_60_thermocycle43pos57.5150714.png |}} |102.21212121212122| {{ :bdev:notemplate_350_60_thermocycle43pos55.5150653.png |}} | 
-G1X228.16666666667Y183.5F3000 +|45|119.06060606060606| {{ :bdev:template_350_60_thermocycle44pos57.5150907.png |}} |100.42424242424242| {{ :bdev:notemplate_350_60_thermocycle44pos55150841.png |}} | 
-G1Z40F3000 +|46|119.93333333333334| {{ :bdev:template_350_60_thermocycle45pos57.5151101.png |}} |101.15151515151516| {{ :bdev:notemplate_350_60_thermocycle45pos55.5151040.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valveoutput +|47|131.53333333333333| {{ :bdev:template_350_60_thermocycle46pos57.5151254.png |}} |102.45454545454545| {{ :bdev:notemplate_350_60_thermocycle46pos57151249.png |}} | 
-valve-1_1_1_1_1_1_1_1-output +|48|123.23333333333333| {{ :bdev:template_350_60_thermocycle47pos58151452.png |}} |106.33333333333333| {{ :bdev:notemplate_350_60_thermocycle47pos56151432.png |}} | 
-sg1e319s6a3_8 +|49|123.39393939393939| {{ :bdev:template_350_60_thermocycle48pos58151646.png |}} |106.9| {{ :bdev:notemplate_350_60_thermocycle48pos56151625.png |}} | 
-//valve-1_1_1_1_1_1_1_1-valvebypass +|50|124.66666666666667| {{ :bdev:template_350_60_thermocycle49pos57.5151834.png |}} |111.26666666666667| {{ :bdev:notemplate_350_60_thermocycle49pos55.5151813.png |}} |
-valve-1_1_1_1_1_1_1_1-bypass +
-//washmodel +
-//motion wash station zh 0 row 1 F3000 +
-G1Z0F3000 +
-G1X235.5Y36F3000 +
-G1Z40F3000 +
-//waste 2 on +
-wasteon +
-//wash 1 on +
-washon +
-G1Z40F3000_10 +
-//wash 1 off +
-washoff +
-//waste 2 off +
-wasteoff +
-//just dry function +
-G1Z0F3000_2 +
-touchdry Z20F3000ZT0T1 +
-G1Z0F3000_2 +
-//brightfieldcam +
-G1X108Y75Z0.0F3000 +
-G1Z55F3000_10 +
-//camsnap 192.168.1.87_11062020123139_afteraspiration   347 50 +
-snap 192.168.1.87_347_50_11062020123139_afteraspiration +
-G1Z55F3000_5 +
-//thermocycle1_photo +
-G1Z0F3000 +
-//thermocycle 1 +
-G1Z0F3000 +
-G1X36Y248F5000_8 +
-//motion heatblock2 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X36Y248F5000 +
-G1Z22F3000 +
-G1Z42F500_15 +
-//motion heatblock1 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X34Y198.5F3000 +
-G1Z22F3000 +
-G1Z42F500_30 +
-//motion PCRcam zh 0 row 1 F3000 +
-G1Z0F3000 +
-G1X5.5Y89.5F5000 +
-G1Z46F3000_2 +
-G1Z0F3000_2 +
-//brightfieldcam +
-G1X108Y75Z0.0F3000 +
-G1Z55F3000_15 +
-//camsnap 192.168.1.87_11062020123139_thcycl   347 50 +
-snap 192.168.1.87_347_50_11062020123139_thcycl1 +
-G1Z55F3000_5 +
-G1Z0F3000 +
-G1Z0F3000 +
-G1X36Y248F5000_8 +
-//thermocycle 2 +
-//motion heatblock2 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X36Y248F5000 +
-G1Z22F3000 +
-G1Z42F500_15 +
-//motion heatblock1 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X34Y198.5F3000 +
-G1Z22F3000 +
-G1Z42F500_30 +
-//motion PCRcam zh 0 row 1 F3000 +
-G1Z0F3000 +
-G1X5.5Y89.5F5000 +
-G1Z46F3000_2 +
-G1Z0F3000_2 +
-//brightfieldcam +
-G1X108Y75Z0.0F3000 +
-G1Z55F3000_15 +
-//camsnap 192.168.1.87_11062020123139_thcycl2   347 50 +
-snap 192.168.1.87_347_50_11062020123139_thcycl2 +
-G1Z55F3000_5 +
-G1Z0F3000 +
-G1Z0F3000 +
-G1X36Y248F5000_8 +
-//thermocycle 3 +
-//motion heatblock2 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X36Y248F5000 +
-G1Z22F3000 +
-G1Z42F500_15 +
-//motion heatblock1 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X34Y198.5F3000 +
-G1Z22F3000 +
-G1Z42F500_30 +
-//motion PCRcam zh 0 row 1 F3000 +
-G1Z0F3000 +
-G1X5.5Y89.5F5000 +
-G1Z46F3000_2 +
-G1Z0F3000_2 +
-//brightfieldcam +
-G1X108Y75Z0.0F3000 +
-G1Z55F3000_15 +
-//camsnap 192.168.1.87_11062020123139_thcycl3   347 50 +
-snap 192.168.1.87_347_50_11062020123139_thcycl3 +
-G1Z55F3000_5 +
-G1Z0F3000 +
-//thermocycle +
-G1Z0F3000 +
-G1X36Y248F5000_8 +
-//thermocycle 4 +
-//motion heatblock2 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X36Y248F5000 +
-G1Z22F3000 +
-G1Z42F500_15 +
-//motion heatblock1 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X34Y198.5F3000 +
-G1Z22F3000 +
-G1Z42F500_30 +
-//motion PCRcam zh 0 row 1 F3000 +
-G1Z0F3000 +
-G1X5.5Y89.5F5000 +
-G1Z46F3000_2 +
-G1Z0F3000_2 +
-//brightfieldcam +
-G1X108Y75Z0.0F3000 +
-G1Z55F3000_15 +
-//camsnap 192.168.1.87_11062020123139_thcycl4   347 50 +
-snap 192.168.1.87_347_50_11062020123139_thcycl4 +
-G1Z55F3000_5 +
-G1Z0F3000 +
-//thermocycle +
-G1X36Y248F5000_8 +
-//thermocycle 5 +
-//motion heatblock2 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X36Y248F5000 +
-G1Z22F3000 +
-G1Z42F500_15 +
-//motion heatblock1 zh 20 row 1 F3000 +
-G1Z0F3000 +
-G1X34Y198.5F3000 +
-G1Z22F3000 +
-G1Z42F500_30 +
-//motion PCRcam zh 0 row 1 F3000 +
-G1Z0F3000 +
-G1X5.5Y89.5F5000 +
-G1Z46F3000_2 +
-G1Z0F3000_2 +
-//brightfieldcam +
-G1X108Y75Z0.0F3000 +
-G1Z55F3000_15 +
-//camsnap 192.168.1.87_11062020123139_thcycl5   347 50 +
-snap 192.168.1.87_347_50_11062020123139_thcycl5 +
-G1Z55F3000_5 +
-//swap volume +
-//valve-1_1_1_1_1_1_1_1-valveinput +
-valve-1_1_1_1_1_1_1_1-input +
-sg1e220s60a30_12 +
-//thermodispense_seg +
-//valve-1_1_1_1_1_1_1_1-valveoutput +
-valve-1_1_1_1_1_1_1_1-output +
-//syringe 316 speed 6 acc 3 +
-sg1e216s6a3_6 +
-//valve-1_1_1_1_1_1_1_1-valvebypass +
-valve-1_1_1_1_1_1_1_1-bypass +
-//camsnap 192.168.1.87_11062020123139_thcycl   347 50 +
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-</code> 
  
pipettetip_thermocycling_imaging.1604719052.txt.gz · Last modified: 2021/08/16 22:21 (external edit)

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