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--- pipettetip_volumetric_calculation [2020/11/02 22:54]
richard
+++ pipettetip_volumetric_calculation [2021/08/16 22:22] (current)
@@ Line 14: / Line 14: @@
+The aspiration process follows the following steps:
+  - Aspirate air 5ul
+  - Aspirate sample 6ul
+  - Aspirate air 3ul
+Here is a video showing the aspiration process:
+{{youtube>VBb4YAIR5CY?&rel=0 | Pipette aspiration for volumetric quantification example}}
+The macro used to do this is posted:
+<code>
+G1Z0F1000_0.5
+//valve-1_1_1_1_1_1_1_1-valveinput
+valve-1_1_1_1_1_1_1_1-input
+//syringe 300 speed 40 acc 20
+sg1e300s40a20_15
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+sg1e305s6a3_10
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//dyeaspmodel
+//dyeaspmodel
+//motion 96well zh 20 row 2 F3000
+G1Z0F3000
+G1X228.16666666667Y183.5F5000_8
+G1Z40F3000
+//motion 96well zh 0 row 2 F500
+G1X228.16666666667Y183.5F500
+G1Z60F500_5
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+//syringe 326 speed 6 acc 3
+sg1e311s6a3_8
+G1Z60F500_2
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 20 row 2 F3000
+G1X228.16666666667Y183.5F3000
+G1Z40F3000
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+sg1e313s6a3_8
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//washmodel
+//motion wash station zh 0 row 1 F3000
+G1Z0F3000
+G1X235.5Y36F3000
+G1Z40F3000
+//waste 2 on
+wasteon
+//wash 1 on
+washon
+G1Z40F3000_30
+//wash 1 off
+washoff
+//waste 2 off
+wasteoff
+//just dry function
+G1Z0F3000_2
+touchdry Z20F3000ZT0T1
+</code>

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