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--- removing_air_bubbles_lines [2020/09/25 00:03]
richard
+++ removing_air_bubbles_lines [2021/08/16 22:22] (current)
@@ Line 2: / Line 2: @@
 ===== LabAutoBox Removing air bubbles from lines =====
+//
+//
+==== Moving pipettes to waste ====
 Air bubbles do form in tubing over time so it is important to remove them before doing liquid handling. The first step to do this is to move the pipette tips to the waste station which is achieved by pressing the "Go to waste" button in [[software_overview|Interactive Mode]].
@@ Line 10: / Line 13: @@
 //
 //
-{{youtube>GeB6QfONg6w}}
+{{youtube>GeB6QfONg6w?&rel=0 }}
 ----
+==== Tubing perfusion when 4-way valve in close position ====
 After the pipettes are in place then you manually push fluid in through them to get the air out of the lines. There is a manual perfusion setup for removing air bubbles from lines. There is a stopcock valve positioned between the PCV and the PCV peristaltic pump.
@@ Line 38: / Line 41: @@
   - Each syringe from the 8-channel syringe pump probably needs to have the luer lock tubing removed (1 at a time and connected to the syringe that is used for drawing fluid out of the PCV). This is to remove some air trapped in the pipette loading module. This will drain out the fluid then next when filled the bubbles are cleared.
+----
+==== Tubing perfusion when 4-way valve in output position ====
+<figure>
+{{ ::labautobox_move_valves_to_output.png?400|Position the 4-way valves to output so that you can clear the bubbles out of the line that connects the multichannel syringe pump to pipettes.}}<caption>**Position the 4-way valves to output so that you can clear the bubbles out of the line that connects the multichannel syringe pump to pipettes.**</caption>
+</figure>
+This will clear the lines from the PCV to the pipettes but you still need to get the air bubbles out between the syringes and valves. To do this, now you need to:
+  - Position the corresponding valve into the output position.
+  - Draw some fluid into the syringe connected to the manual valve that draws fluid from the PCV.
+  - Disconnect the tubing from one of the multichannel syringes and put that end in a bottle and disconnect the corresponding tubing to pipette and attach the fluid filled syringe.
+  - Perfuse until all air bubbles are out and attach to multichannel syringe pump first, put valve into bypass position, then attach the other end of tubing to the corresponding pipette.
+----
+<figure>
+{{ ::labautobox_attach_filled_syringe_to_one_syringepump_channel_v2.png?400|To remove the bubbles coming out of the multichannel syringe pump to the pipettes. You need to fill up a syringe with fluid, disconnect the tubing from the pipettes, disconnect the corresponding tubing that is connected to one of the syringes in the multichannel syringe pump, connect that end to a fluid reservoir (like a bottle) then push bubbles out.}}<caption>**To remove the bubbles coming out of the multichannel syringe pump to the pipettes. You need to fill up a syringe with fluid, disconnect the tubing from the pipettes, disconnect the corresponding tubing that is connected to one of the syringes in the multichannel syringe pump, connect that end to a fluid reservoir (like a bottle) then push bubbles out.**</caption>
+</figure>

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