LabAutobox controls the temperature of 2 thermo blocks which the pipettes move into afterwards they can be imaged. To show the concept, the tips are incubating for only 5 seconds per block in the video.

The code works something like this:

1. First you need to set the thermo block heaters to the temperature range that you want to run the thermocycling (ie., 94C denaturation, 60 extension).

//heatblockstemp block1 60 block2 100
M104 T0 S60
M104 T1 S100

1. Then you just assemble the macro steps to form the routine, it would look something like this for one cycle

//thermocycler_imaging
//motion heatblock1 zh 0 row 1 F3000
G1Z0F3000
G1X34Y198.5Z0F3000_6
G1Z32F2000
G1Z42F400_30
//motion heatblock2 zh 0 row 1 F3000
G1Z0F3000
G1X36Y248Z0F3000_5
G1Z32F2000_2
G1Z42F400_180
G1Z0F3000
//motion PCRcam zh 0 row 1 F3000
G1Z0F3000
G1X5.5Y83.5F3000
G1Z46F3000
//camsnap 192.168.1.89_09302020070456_pipetteimaging   400 70
snap 192.168.1.89_400_70_09302020070456_pipetteimaging