LabAutobox controls the temperature of 2 thermo blocks which the pipettes move into afterwards they can be imaged. To show the concept, the tips are incubating for only 5 seconds per block in the video.

The code works something like this:

1. First you need to set the thermo block heaters to the temperature range that you want to run the thermocycling (ie., 94C denaturation, 60 extension).

//heatblockstemp block1 60 block2 100
M104 T0 S60
M104 T1 S100

1. Then you just assemble the macro steps to form the routine, it would look something like this for one cycle

//thermocycler_imaging
//motion heatblock1 zh 0 row 1 F3000
G1Z0F3000
G1X34Y198.5Z0F3000_6
G1Z32F2000
G1Z42F400_30
//motion heatblock2 zh 0 row 1 F3000
G1Z0F3000
G1X36Y248Z0F3000_5
G1Z32F2000_2
G1Z42F400_180
G1Z0F3000
//motion PCRcam zh 0 row 1 F3000
G1Z0F3000
G1X5.5Y83.5F3000
G1Z46F3000
//camsnap 192.168.1.89_09302020070456_pipetteimaging   400 70
snap 192.168.1.89_400_70_09302020070456_pipetteimaging

cycle 1	cycle 2	cycle 3	cycle 4	cycle 5	cycle 6	cycle 8	cycle 9	cycle 10	cycle 11
cycle 12	cycle 13	cycle 14	cycle 15
cycle 16
cycle 17	cycle 18	cycle 19	cycle 20	cycle 21
cycle 22	cycle 23	cycle 24	cycle 25	cycle 26
cycle 27	cycle 28	cycle 29	cycle 30	cycle 31
cycle 32
cycle 34	cycle 35	cycle 36
cycle 37	cycle 38	cycle 39	cycle 40	cycle 41
cycle 42	cycle 43	cycle 44	cycle 45