LabBot3D-Pi: Imaging tools and techniques
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The LabBot3D-Pi software allow for both individual camera adjustment and picture taking and for running the camera in a macro in synchronization with liquid handling methods.
LabBot3D-Pi - imaging tools and techniques
A camera is very useful both for calibrating positioning and for being used as a general purpose data collection tool. Here shown are a couple of examples where a Raspberry Pi camera is used for visualizing microwells in 96 well plates and pipette tips in a 8 channel format. There is a RaspberryPi Zero that is connected to the z drive linear encoder end stop flag so the computer moves up and down along with the camera. The camera is conveniently adjustable and its possible to hook up lighting schemes if needed. Also the set up is not limited to a Raspberry Pi camera, there are other cameras available as well (OpenMV and Imaging Source - which can be triggered with an analog signal ... please inquire).
This is a RaspberryPi camera case that can be connected with different focal distance lenses, also
. The camera is attached to arms that allows for pivot points and the attachment module allows the camera to slide in the X direction for adjustment. The attachment module is large enough to connect another camera and/or light source.
For visualizing wells, the camera can be set up to work with a focal distance 25mm lens. This can be useful for doing ELISA colormetric well analysis or for doing microarray in wells, or analyzing spots (like Elispots) since the resolution is around 5 microns per pixel.
For visualizing tips, a focal distance of 8mm is recommended. The lens mount is a little smaller than the F25 lens. In this case if wanted to see the tips the camera could be stationary (not attached to the pipetting head). Image processing can be set up to see the tips and measure the volume in the tips if there is a good enough background to give sufficient contrast.
The deck is set up to be able to read every well for 8 plates
Example of imaged wells for a 96 well microwell plate. Rows 11 and 12 are resting on the aluminum extrusion that is causing the discoloration. It is possible to have the plate rest on a dark surface to resolve the problem.