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--- microwell_aspiration [2020/09/28 16:41]
richard
+++ microwell_aspiration [2021/08/16 22:22] (current)
@@ Line 1: / Line 1: @@
 ====== Microwell aspiration ======
+In order to accurate aspirate small volumes it is important to make sure the air bubbles are mostly out of the lines and there are 2 pages that describe how to do this:
+  - [[removing_air_bubbles_lines|Removing air bubbles]] - This technique allows you to manually perfuse the lines with a syringe.
+  - [[putting_together_pipette_flushing_macro|Putting together a pipette flushing macro]] - This uses the multichannel syringe pump to clean out the lines
+{{youtube>Gi_h4BmyQI4?&rel=0 | 10 microliter aspiration macro using airgap}}
+{{youtube>Z4cRSgj_dKw?&rel=0 | 10microliter aspiration example}}
+----
+<code>
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 20 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z33F3000
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+//syringe 50 speed 40 acc 20
+sg1e50s40a20_1
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 0 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z53F3000
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+//syringe 60 speed 20 acc 10
+sg1e60s20a10_8
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 30 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z23F3000
+</code>
+----
+===== Volume aspiration benchmarking =====
+Here is some benchmarking data comparing 1,2.5,5 and 10 microliters. Benchmarking was done using Rainin LTS RT-L10 tips
+{{:volume_titration.png?400 |Volume titration }}
+<code>
+//aspiration_model_airgap
+//flushlines
+//flushpipettes
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion waste station zh 0 row 1 F3000
+G1Z0F3000
+G1X235.5Y24F3000
+G1Z8F3000_5
+//valve-1_1_1_1_1_1_1_1-valveinput
+valve-1_1_1_1_1_1_1_1-input
+//syringe 500 speed 40 acc 20
+sg1e500s40a20_20
+//valve-1_1_1_1_1_1_1_1-valveoutput
+wasteon
+valve-1_1_1_1_1_1_1_1-output
+//syringe 0 speed 40 acc 20
+sg1e0s40a20_20
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//just dry function
+touchdry Z13F3000ZT0T0.4
+wasteoff
+G1Z0F3000
+G1X235.5Y24F3000
+G1Z8F3000_5
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//aspiration_model_airgap
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 20 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z27F3000_4
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+//syringe 50 speed 40 acc 20
+sg1e50s40a20_7
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 0 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z53F3000
+valve-1_1_1_1_1_1_1_1-output
+G1Z52F3000_2
+//syringe 60 speed 20 acc 10
+sg1e51s5a2_7
+G1Z53F3000_2
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 30 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z17F3000
+restart
+</code>

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