microwell_aspiration
Microwell aspiration
In order to accurate aspirate small volumes it is important to make sure the air bubbles are mostly out of the lines and there are 2 pages that describe how to do this:
- Removing air bubbles - This technique allows you to manually perfuse the lines with a syringe.
- Putting together a pipette flushing macro - This uses the multichannel syringe pump to clean out the lines
//valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion 96well zh 20 row 1 F3000 G1Z0F3000 G1X171.48Y176.5F3000 G1Z33F3000 //valve-1_1_1_1_1_1_1_1-valveoutput valve-1_1_1_1_1_1_1_1-output //syringe 50 speed 40 acc 20 sg1e50s40a20_1 //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion 96well zh 0 row 1 F3000 G1Z0F3000 G1X171.48Y176.5F3000 G1Z53F3000 //valve-1_1_1_1_1_1_1_1-valveoutput valve-1_1_1_1_1_1_1_1-output //syringe 60 speed 20 acc 10 sg1e60s20a10_8 //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion 96well zh 30 row 1 F3000 G1Z0F3000 G1X171.48Y176.5F3000 G1Z23F3000
Volume aspiration benchmarking
Here is some benchmarking data comparing 1,2.5,5 and 10 microliters. Benchmarking was done using Rainin LTS RT-L10 tips
//aspiration_model_airgap //flushlines //flushpipettes //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion waste station zh 0 row 1 F3000 G1Z0F3000 G1X235.5Y24F3000 G1Z8F3000_5 //valve-1_1_1_1_1_1_1_1-valveinput valve-1_1_1_1_1_1_1_1-input //syringe 500 speed 40 acc 20 sg1e500s40a20_20 //valve-1_1_1_1_1_1_1_1-valveoutput wasteon valve-1_1_1_1_1_1_1_1-output //syringe 0 speed 40 acc 20 sg1e0s40a20_20 //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //just dry function touchdry Z13F3000ZT0T0.4 wasteoff G1Z0F3000 G1X235.5Y24F3000 G1Z8F3000_5 //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //aspiration_model_airgap //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion 96well zh 20 row 1 F3000 G1Z0F3000 G1X171.48Y176.5F3000 G1Z27F3000_4 //valve-1_1_1_1_1_1_1_1-valveoutput valve-1_1_1_1_1_1_1_1-output //syringe 50 speed 40 acc 20 sg1e50s40a20_7 //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion 96well zh 0 row 1 F3000 G1Z0F3000 G1X171.48Y176.5F3000 G1Z53F3000 valve-1_1_1_1_1_1_1_1-output G1Z52F3000_2 //syringe 60 speed 20 acc 10 sg1e51s5a2_7 G1Z53F3000_2 //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion 96well zh 30 row 1 F3000 G1Z0F3000 G1X171.48Y176.5F3000 G1Z17F3000 restart
microwell_aspiration.txt · Last modified: 2021/08/16 22:22 (external edit)
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