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--- microwell_aspiration [2020/09/29 00:45]
richard
+++ microwell_aspiration [2021/08/16 22:22] (current)
@@ Line 6: / Line 6: @@
+{{youtube>Gi_h4BmyQI4?&rel=0 | 10 microliter aspiration macro using airgap}}
-https://youtu.be/KcYXge0HYjU
+{{youtube>Z4cRSgj_dKw?&rel=0 | 10microliter aspiration example}}
-https://youtu.be/Z4cRSgj_dKw
+----
 <code>
-//aspiration_model_airgap
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 20 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z33F3000
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+//syringe 50 speed 40 acc 20
+sg1e50s40a20_1
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 0 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z53F3000
+//valve-1_1_1_1_1_1_1_1-valveoutput
+valve-1_1_1_1_1_1_1_1-output
+//syringe 60 speed 20 acc 10
+sg1e60s20a10_8
 //valve-1_1_1_1_1_1_1_1-valvebypass
 valve-1_1_1_1_1_1_1_1-bypass
@@ Line 18: / Line 40: @@
 G1Z0F3000
 G1X171.48Y176.5F3000
-G1Z17F3000
+G1Z23F3000
+</code>
+----
+===== Volume aspiration benchmarking =====
+Here is some benchmarking data comparing 1,2.5,5 and 10 microliters. Benchmarking was done using Rainin LTS RT-L10 tips
+{{:volume_titration.png?400 |Volume titration }}
+<code>
+//aspiration_model_airgap
+//flushlines
+//flushpipettes
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion waste station zh 0 row 1 F3000
+G1Z0F3000
+G1X235.5Y24F3000
+G1Z8F3000_5
+//valve-1_1_1_1_1_1_1_1-valveinput
+valve-1_1_1_1_1_1_1_1-input
+//syringe 500 speed 40 acc 20
+sg1e500s40a20_20
+//valve-1_1_1_1_1_1_1_1-valveoutput
+wasteon
+valve-1_1_1_1_1_1_1_1-output
+//syringe 0 speed 40 acc 20
+sg1e0s40a20_20
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//just dry function
+touchdry Z13F3000ZT0T0.4
+wasteoff
+G1Z0F3000
+G1X235.5Y24F3000
+G1Z8F3000_5
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//aspiration_model_airgap
+//valve-1_1_1_1_1_1_1_1-valvebypass
+valve-1_1_1_1_1_1_1_1-bypass
+//motion 96well zh 20 row 1 F3000
+G1Z0F3000
+G1X171.48Y176.5F3000
+G1Z27F3000_4
 //valve-1_1_1_1_1_1_1_1-valveoutput
 valve-1_1_1_1_1_1_1_1-output
 //syringe 50 speed 40 acc 20
-sg1e50s40a20_10
+sg1e50s40a20_7
 //valve-1_1_1_1_1_1_1_1-valvebypass
 valve-1_1_1_1_1_1_1_1-bypass
@@ Line 28: / Line 96: @@
 G1Z0F3000
 G1X171.48Y176.5F3000
-G1Z47F3000
+G1Z53F3000
+valve-1_1_1_1_1_1_1_1-output
+G1Z52F3000_2
+//syringe 60 speed 20 acc 10
+sg1e51s5a2_7
+G1Z53F3000_2
 //valve-1_1_1_1_1_1_1_1-valvebypass
 valve-1_1_1_1_1_1_1_1-bypass
-//motion 96well zh 40 row 1 F3000
+//motion 96well zh 30 row 1 F3000
 G1Z0F3000
 G1X171.48Y176.5F3000
-G1Z7F3000
+G1Z17F3000
+restart
 </code>

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