Flushing the pipette tips is a good way to assess that they air bubbles are out of the lines everything is working well. Here are steps on how to build a flushing macro using the “Build Macro” page (introduced here), which will be saved as a custom macro. Generally, the steps involved are as followed:
Here is a video that explains how to compile the macro.
Here is the macro code.
//valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //motion waste station zh 0 row 1 F3000 G1Z0F3000 G1X235.5Y24F3000 G1Z12F3000 //valve-1_1_1_1_1_1_1_1-valveinput valve-1_1_1_1_1_1_1_1-input //syringe 500 speed 40 acc 20 sg1e500s40a20_17 G1Z12F3000_2 //waste 2 on wasteon //valve-1_1_1_1_1_1_1_1-valveoutput valve-1_1_1_1_1_1_1_1-output //syringe 0 speed 40 acc 20 sg1e0s40a20_17 //valve-1_1_1_1_1_1_1_1-valvebypass valve-1_1_1_1_1_1_1_1-bypass //just dry function touchdry Z9.5F3000ZT0T0.1 //waste 2 off wasteoff
Here is video showing the process