In order to accurate aspirate small volumes it is important to make sure the air bubbles are mostly out of the lines and there are 2 pages that describe how to do this:

1. Removing air bubbles - This technique allows you to manually perfuse the lines with a syringe.
2. Putting together a pipette flushing macro - This uses the multichannel syringe pump to clean out the lines

https://youtu.be/KcYXge0HYjU https://youtu.be/Z4cRSgj_dKw

//aspiration_model_airgap
//valve-1_1_1_1_1_1_1_1-valvebypass
valve-1_1_1_1_1_1_1_1-bypass
//motion 96well zh 30 row 1 F3000
G1Z0F3000
G1X171.48Y176.5F3000
G1Z17F3000
//valve-1_1_1_1_1_1_1_1-valveoutput
valve-1_1_1_1_1_1_1_1-output
//syringe 50 speed 40 acc 20
sg1e50s40a20_10
//valve-1_1_1_1_1_1_1_1-valvebypass
valve-1_1_1_1_1_1_1_1-bypass
//motion 96well zh 0 row 1 F3000
G1Z0F3000
G1X171.48Y176.5F3000
G1Z47F3000
//valve-1_1_1_1_1_1_1_1-valvebypass
valve-1_1_1_1_1_1_1_1-bypass
//motion 96well zh 40 row 1 F3000
G1Z0F3000
G1X171.48Y176.5F3000
G1Z7F3000